SLIMamp Overview

Fast

  • Ready-to-use targeted NGS panels
  • Single tube workflow

Scalable

  • Single tube design for highly multiplexed panels
  • Compatible with automation

On Target

  • Sensitive, specific primer design for clinically relevant regions
  • Generate libraries from challenging samples and high GC regions
  • Results from nanograms of DNA input

Flexible

  • Compatible with DNA from multiple sources, including FFPE, blood, isolated genomic DNA as well as ctDNA
  • Panels for somatic and germline variants

SLIMamp Technology Workflow

Optimized workflow to enhance on-target capture

    • Tissue (FFPE, Fresh Frozen, ctDNA)
    • Multiplex PCR assay
    • Targeted primers designed for clinically relevant regions
    • Chemistry overcomes sample quality issues and increases overall recovery of high GC rich regions
    • Target (desired) product is amplified preferentially while short (non-target) amplicons are effectively blocked
    • Eliminates off target reads, increasing percentage of mapped target reads

Example of SLIMamp Variant Stats (BRCA Panel )

A
Unique Variants
Type Count Notes
Insertion 4 1 bp
Deletion 4 1-4 bp, 11 bp, and 40 bp
SNV 47  
Total 66  
B
Variant Detection from 34 unique samples with full ROI region
validated by at least one independent method
Variant Detected Variant not Detected
True Positive (TP) 409 SNV_HET 234 False Negative (FN) 0
SNV_HOM 153
Indels_HET (1-40 bp) 22
False Positive (TP) 0   True Negative (TN)* 603737
*TN= Size of ROI (17769 bp) x Total Samples (34) - Total True positives in all samples
C
Analytical sensitivity and specificity
Statistic Formula Value 95% Cl
Sensitivity TP/(TP+FN) 100.00% 99.10% to 100.00%
Specificity TP/(TP+FN) 100.00% 100.00% to 100.00%
A
Unique Variants
Type Count Notes
Insertion 4 1 bp
Delection 4 1-4 bp, 11 bp, and 40 bp
SNV 47  
Total 66  
B
Variant Detection from 34 unique samples with full ROI region
validated by at least one independent method
Variant Detected Variant not Detected
True Positive (TP) 409 SNV_HET 234 False Negative (FN) 0
SNV_HOM 153
Indels_HET (1-40 bp) 22
False Positive (TP) 0   True Negative (TN)* 603737
*TN= Size of ROI (17769 bp) x Total Samples (34) - Total True positives in all samples
C
Analytical sensitivity and specificity
Statistic Formula Value 95% Cl
Sensitivity TP/(TP+FN) 100.00% 99.10% to 100.00%
Sensitivity TP/(TP+FN) 100.00% 99.10% to 100.00%