Bioinformatics that help you find the variant in the haystack
Streamline your bioinformatics analysis with PiVAT®
The Pillar® Variant Analysis Toolkit (PiVAT) is a bioinformatic secondary analysis pipeline that is optimized for SLIMamp® library prep chemistry, making data analysis and interpretation straightforward and effective.
Rapid
Fast, actionable results
The PiVAT platform is maximized for efficiency, delivering fast processing times with minimal computing resources
Flexible
Comprehensive variant detection
The PiVAT platform captures virtually every type of variant: SNVs, CNVs, ITDs, indels, delins, RNA fusions, and more.
Robust
Sensitivity you can count on
Overlapping, paired-end amplicons help identify and filter errors for calls as low as 1% VAF (<1% for pillarHS panels)
PiVAT bioinformatics workflow
PiVAT delivers reliable answers even for damaged samples by reducing sequencing errors
Formalin-compromised reference standards (Horizon Discovery) were evaluated using the ONCO/Reveal Lung and Colon Cancer Panel. N=10; error bars=standard deviation
x-axis: ONCO/Reveal Multi-Cancer target sequence of 25kb. y-axis: Error rate. Orange: Frequency of ordinary recurring errors (Q scores of 20 and above). Green: PiVAT error rate
PiVAT was built to go hand-in-hand with Pillar’s ampPD™ primer design platform and SLIMamp® chemistry–so the entire NGS workflow is seamlessly integrated, even for custom genomic targets.
ampPD™
Primer design platform for reliable amplicon specificity
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