Pillar® ONCO/Reveal™ TP53 Panel

The ONCO/Reveal TP53 Panel covers the entire CDS of the TP53 tumor suppressor gene. The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology for efficient single-tube target enrichment.

Panel highlights

Full TP53 CDS plus flanking sequences (19 amplicons) 

Sample to library in as little as 5 hours (2hrs hands on) 

As little as 5ng input DNA

100% coverage uniformity at 0.2x mean

For research use only. Not for use in diagnostic procedures.

Panel specifications

Enrichment chemistry Multiplex PCR using tiled amplicons
Number of pools 1 pool
Number of genes/amplicons 1/19
Number of targets Full CDS plus 10bp flanking introns; 2.1k total bases covered
Variant types SNVs, small and medium indels
Average amplicon size 149bp (138bp-180bp range)
Input DNA range 5ng to 50ng
Sample types DNA from tissue or blood; cfDNA; FFPE
Mapping rate 95.9% ± 2.1%
% on-target aligned reads 94.0% ± 3.3%
Coverage uniformity (% targets with >0.2X mean coverage) 100%
Total assay time (from DNA to sequencer) <8 hours
Sequencing platforms Illumina®; assay is coming soon for MGISEQ™ and Ion Torrent™ platforms

See the data

Automated Primer Design for Single-Tube Multiplex PCR for Tiled Amplicon Resequencing –TP53 Assay Design and Characterization as a Pilot Study

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Pillar technology

Pillar provides a seamlessly integrated NGS workflow, from panel design to data analysis.

ampPD™

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Primer design platform for reliable amplicon specificity

SLIMamp®

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Enrichment chemistry that enables single-tube amplification

PiVAT®

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Bioinformatic tools that deliver sensitive, robust variant calls

Contact an expert

Contact us for technical information or to get a quote

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