Pillar® ONCO/Reveal™ TP53 Panel

The ONCO/Reveal TP53 Panel covers the entire CDS of the TP53 tumor suppressor gene. The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology for efficient single-tube target enrichment.

Panel highlights

Full TP53 CDS plus flanking sequences (19 amplicons) 

Sample to library in as little as 5 hours (2hrs hands on) 

As little as 5ng input DNA

100% coverage uniformity at 0.2x mean

For research use only. Not for use in diagnostic procedures.

Panel specifications

Enrichment chemistry Multiplex PCR using tiled amplicons
Number of pools 1 pool
Number of genes/amplicons 1/19
Number of targets Full CDS plus 10bp flanking introns; 2.1k total bases covered
Variant types SNVs, small and medium indels
Average amplicon size 149bp (138bp-180bp range)
Input DNA range 5ng to 50ng
Sample types DNA from tissue or blood; cfDNA; FFPE
Mapping rate 95.9% ± 2.1%
% on-target aligned reads 94.0% ± 3.3%
Coverage uniformity (% targets with >0.2X mean coverage) 100%
Total assay time (from DNA to sequencer) <8 hours
Sequencing platforms Illumina®; assay is coming soon for MGISEQ™ and Ion Torrent™ platforms

See the data

Automated Primer Design for Single-Tube Multiplex PCR for Tiled Amplicon Resequencing –TP53 Assay Design and Characterization as a Pilot Study


Pillar technology

Pillar provides a seamlessly integrated NGS workflow, from panel design to data analysis.


icon of ampPD primer as located on DNA

Primer design platform for reliable amplicon specificity


test tube icon

Enrichment chemistry that enables single-tube amplification


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Bioinformatic tools that deliver sensitive, robust variant calls

Contact an expert

Contact us for technical information or to get a quote

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