Pillar® ONCO/Reveal™ TP53 Panel
The ONCO/Reveal TP53 Panel covers the entire CDS of the TP53 tumor suppressor gene. The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology for efficient single-tube target enrichment.
Panel highlights
Full TP53 CDS plus flanking sequences (19 amplicons)
Sample to library in as little as 5 hours (2hrs hands on)
As little as 5ng input DNA
100% coverage uniformity at 0.2x mean
For research use only. Not for use in diagnostic procedures.
Panel specifications
| Enrichment chemistry | Multiplex PCR using tiled amplicons |
| Number of pools | 1 pool |
| Number of genes/amplicons | 1/19 |
| Number of targets | Full CDS plus 10bp flanking introns; 2.1k total bases covered |
| Variant types | SNVs, small and medium indels |
| Average amplicon size | 149bp (138bp-180bp range) |
| Input DNA range | 5ng to 50ng |
| Sample types | DNA from tissue or blood; cfDNA; FFPE |
| Mapping rate | 95.9% ± 2.1% |
| % on-target aligned reads | 94.0% ± 3.3% |
| Coverage uniformity (% targets with >0.2X mean coverage) | 100% |
| Total assay time (from DNA to sequencer) | <8 hours |
| Sequencing platforms | Illumina®; assay is coming soon for MGISEQ™ and Ion Torrent™ platforms |
See the data
AMP 2018 - TP53 Panel Poster
Automated Primer Design for Single-Tube Multiplex PCR for Tiled Amplicon Resequencing –TP53 Assay Design and Characterization as a Pilot Study
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