Pillar® ONCO/Reveal™ TP53 Panel
The ONCO/Reveal TP53 Panel covers the entire CDS of the TP53 tumor suppressor gene. The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology for efficient single-tube target enrichment.
Full TP53 CDS plus flanking sequences (19 amplicons)
Sample to library in as little as 5 hours (2hrs hands on)
As little as 5ng input DNA
100% coverage uniformity at 0.2x mean
For research use only. Not for use in diagnostic procedures.
|Enrichment chemistry||Multiplex PCR using tiled amplicons|
|Number of pools||1 pool|
|Number of genes/amplicons||1/19|
|Number of targets||Full CDS plus 10bp flanking introns; 2.1k total bases covered|
|Variant types||SNVs, small and medium indels|
|Average amplicon size||149bp (138bp-180bp range)|
|Input DNA range||5ng to 50ng|
|Sample types||DNA from tissue or blood; cfDNA; FFPE|
|Mapping rate||95.9% ± 2.1%|
|% on-target aligned reads||94.0% ± 3.3%|
|Coverage uniformity (% targets with >0.2X mean coverage)||100%|
|Total assay time (from DNA to sequencer)||<8 hours|
|Sequencing platforms||Illumina®; assay is coming soon for MGISEQ™ and Ion Torrent™ platforms|
Pillar provides a seamlessly integrated NGS workflow, from panel design to data analysis.
Primer design platform for reliable amplicon specificity
Enrichment chemistry that enables single-tube amplification
Bioinformatic tools that deliver sensitive, robust variant calls