Pillar® ONCO/Reveal™ Solid Tumor Panel

The ONCO/Reveal Solid Tumor Panel is a robust 47-gene assay that simultaneously tests for key mutations present in solid tumors, including NSCLC, colorectal, pancreatic, GIST, thyroid cancer, melanomas and gliomas. Additionally, genes with potential importance in immuno-oncology such as POLD1 and POLE are analyzed. The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology for efficient single-tube target enrichment.

Panel highlights

NSCLC, colorectal, pancreatic, GIST, thyroid, melanomas, and gliomas

Full CDS coverage in 3 genes, hotspots in 44 additional genes 

As little as 2.5ng input DNA 

>98% coverage uniformity at 0.2x mean coverage

For research use only. Not for use in diagnostic procedures.

Panel targets

AKT1 CYSLTR2 FBXW7 GNAS KIT PDGFRA PTPN11 SRSF2
ALK DDR2 FGFR1 H3F3A KRAS PIK3CA RAC1 STK11
ARAF EGFR FGFR2 HIST1H3B MAP2K1 PLCB4 RAF1 TERT
BRAF EIF1AX FGFR3 HRAS MET POLD1 RET TP53
CDKN2A ERBB2 GNA11 IDH1 NRAS POLE SF3B1 TSHR
CTNNB1 ERBB4 GNAQ IDH2 NTRK1 PTEN SMAD4

Panel specifications

Enrichment chemistry Multiplex PCR using tiled amplicons
Number of pools 1 pool
Number of genes/amplicons 47/222
Number of targets 24kb total coverage; full CDS coverage of 3 genes; hotspots in 44 genes
Variant types SNVs, small and medium indels
Average amplicon size 152bp
Recommended DNA input range 2.5ng to 60ng (10ng recommended)
Sample types DNA from tissue or blood; cfDNA; FFPE
Mapping rate 98.7% ± 0.9%
% on-target aligned reads 98.7% ± 0.3%
Coverage uniformity (% targets with >0.2X mean coverage) 98.7% ± 0.9%
Total assay time (from DNA to sequencer) <8 hours
Sequencing platforms Illumina®, MGISEQ™ and Ion Torrent™ platforms

Pillar technology

Pillar provides a seamlessly integrated NGS workflow, from panel design to data analysis.

ampPD™

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Primer design platform for reliable amplicon specificity

SLIMamp®

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Enrichment chemistry that enables single-tube amplification

PiVAT®

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Bioinformatic tools that deliver sensitive, robust variant calls

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