Pillar® ONCO/Reveal™ Solid Tumor Panel
The ONCO/Reveal Solid Tumor Panel is a robust 47-gene assay that simultaneously tests for key mutations present in solid tumors, including NSCLC, colorectal, pancreatic, GIST, thyroid cancer, melanomas and gliomas. Additionally, genes with potential importance in immuno-oncology such as POLD1 and POLE are analyzed. The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology for efficient single-tube target enrichment.
NSCLC, colorectal, pancreatic, GIST, thyroid, melanomas, and gliomas
Full CDS coverage in 3 genes, hotspots in 44 additional genes
As little as 2.5ng input DNA
>98% coverage uniformity at 0.2x mean coverage
For research use only. Not for use in diagnostic procedures.
|Enrichment chemistry||Multiplex PCR using tiled amplicons|
|Number of pools||1 pool|
|Number of genes/amplicons||47/222|
|Number of targets||24kb total coverage; full CDS coverage of 3 genes; hotspots in 44 genes|
|Variant types||SNVs, small and medium indels|
|Average amplicon size||152bp|
|Recommended DNA input range||2.5ng to 60ng (10ng recommended)|
|Sample types||DNA from tissue or blood; cfDNA; FFPE|
|Mapping rate||98.7% ± 0.9%|
|% on-target aligned reads||98.7% ± 0.3%|
|Coverage uniformity (% targets with >0.2X mean coverage)||98.7% ± 0.9%|
|Total assay time (from DNA to sequencer)||<8 hours|
|Sequencing platforms||Illumina®, MGISEQ™ and Ion Torrent™ platforms|
Pillar provides a seamlessly integrated NGS workflow, from panel design to data analysis.
Primer design platform for reliable amplicon specificity
Enrichment chemistry that enables single-tube amplification
Bioinformatic tools that deliver sensitive, robust variant calls