Pillar® ONCO/Reveal™ Myeloid Panel

The ONCO/Reveal Myeloid Panel is a robust NGS assay that interrogates 58 genes of interest most relevant to myeloid cancer. The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology for efficient single-tube target enrichment.

Panel highlights

CDS of 18 genes plus hotspots in 40

Detects internal tandem duplicates (ex. FLT3) as well as SNVs and indels

Sample to library in as little as 5 hours (2hrs hands on)

High coverage uniformity even in CG rich regions

For research use only. Not for use in diagnostic procedures.

Panel targets

ABL1	BRAF	CEBPA *	ETV6 *	HRAS	KDM6A *	NPM1	PTEN	SMC1A	TP53 *
ANKRD26	CALR	CSF3R	EZH2 *	IDH1	KIT	NRAS	PTPN11	SMC3	U2AF1
ASXL1	CBL	CUX1	FLT3	IDH2	KMT2A	PDGFRA	RAD21 *	SRSF2	WT1
ATRX	CBLB	DDX41	GATA1	IKZF1 *	KRAS	PHF6 *	RUNX1 *	STAG1	ZRSR2 *
BCOR *	CBLC	DNMT3A *	GATA2 *	JAK2	MPL	PIGA *	SETBP1	STAG2 *
BCORL1 *	CDKN2A *	ETNK1	GNAS	JAK3	NF1	PPM1D	SF3B1	TET2 *

Full CDS coverage in genes indicated by *

Panel specifications

Enrichment chemistry	Multiplex PCR using tiled amplicons
Number of pools	1 pool
Number of genes/amplicons	58/766
Number of targets	Full CDS coverage of 18 genes plus hotspots on 40 additional genes
Variant types	SNVs, indels, ITD (internal tandem duplicates) for FLT3
Average amplicon size	215bp
Recommended DNA input range	10ng to 80ng (20ng recommended)
Sample types	Whole blood, cfDNA, PBMCs
Mapping rate	99.6% ± 0.2%
% on-target aligned reads	92.0% ± 5.3%
Coverage uniformity (% targets with >0.2X mean coverage)	96.8% ± 1.0%
Total assay time (from DNA to sequencer)	<8 hours
Sequencing platforms	Illumina®, MGISEQ™, and Ion Torrent™