Pillar® ONCO/Reveal™ Myeloid Panel

The ONCO/Reveal Myeloid Panel is a robust NGS assay that interrogates 58 genes of interest most relevant to myeloid cancer. The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology for efficient single-tube target enrichment.

Panel highlights

CDS of 18 genes plus hotspots in 40

Detects internal tandem duplicates (ex. FLT3) as well as SNVs and indels 

Sample to library in as little as 5 hours (2hrs hands on)

High coverage uniformity even in CG rich regions 
 

For research use only. Not for use in diagnostic procedures.

Panel targets

ABL1 BRAF CEBPA * ETV6 * HRAS KDM6A * NPM1 PTEN SMC1A TP53 *
ANKRD26 CALR CSF3R EZH2 * IDH1 KIT NRAS PTPN11 SMC3 U2AF1
ASXL1 CBL CUX1 FLT3 IDH2 KMT2A PDGFRA RAD21 * SRSF2 WT1
ATRX CBLB DDX41 GATA1 IKZF1 * KRAS PHF6 * RUNX1 * STAG1 ZRSR2 *
BCOR * CBLC DNMT3A * GATA2 * JAK2 MPL PIGA * SETBP1 STAG2 *
BCORL1 * CDKN2A * ETNK1 GNAS JAK3 NF1 PPM1D SF3B1 TET2 *
Full CDS coverage in genes indicated by *

Panel specifications

Enrichment chemistry Multiplex PCR using tiled amplicons
Number of pools 1 pool
Number of genes/amplicons 58/766
Number of targets Full CDS coverage of 18 genes plus hotspots on 40 additional genes
Variant types SNVs, indels, ITD (internal tandem duplicates) for FLT3
Average amplicon size 215bp
Recommended DNA input range 10ng to 80ng (20ng recommended)
Sample types Whole blood, cfDNA, PBMCs
Mapping rate 99.6% ± 0.2%
% on-target aligned reads 92.0% ± 5.3%
Coverage uniformity (% targets with >0.2X mean coverage) 96.8% ± 1.0%
Total assay time (from DNA to sequencer) <8 hours
Sequencing platforms Illumina®, MGISEQ™, and Ion Torrent™

See the data

Development and Characterization of ONCO/Reveal Myeloid Panel; A Single-Tube, Multiplex-PCR Based NGS Assay with 739 Tiled Amplicons

Download

Detection of Internal Tandem Duplications in the FLT3 gene using PiVAT Software

Download

Pillar technology

Pillar provides a seamlessly integrated NGS workflow, from panel design to data analysis.

ampPD™

icon of ampPD primer as located on DNA

Primer design platform for reliable amplicon specificity

SLIMamp®

test tube icon

Enrichment chemistry that enables single-tube amplification

PiVAT®

PiVat icon

Bioinformatic tools that deliver sensitive, robust variant calls

Contact an expert

Contact us for technical information or to get a quote

WP Feedback

Dive straight into the feedback!
Login below and you can start commenting using your own user instantly

Scroll to Top