Pillar® ONCO/Reveal™ Multi-Cancer v4 with CNV Panel

The ONCO/Reveal Multi-Cancer v4 with CNV Panel is a robust NGS assay that interrogates genes of interest across multiple solid tumor cancer types. The assay uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology, a tiled amplicon-based library prep chemistry for efficient single-tube target enrichment. In addition to SNVs and indels, the assay detects CNVs in 14 genes.

Panel highlights

Assess multiple solid tumor cancer types 

Hotspots in 60 genes

Detect CNVs in addition to SNVs and indels 

As little as 5ng input DNA

For research use only. Not for use in diagnostic procedures.

Panel targets

ABL1 CDKN2A FBXW7 GNAS KIT NPM1 PTPN11 SRC
AKT1 CSF1R FGFR1 HNF1A KRAS NRAS RAC1 STK11
ALK CTNNB1 FGFR2 HRAS MAP2K1 NTRK1 RB1 TP53
APC DDR2 FGFR3 IDH1 MET* NTRK2 RET VHL
ATM EGFR* FLT3 IDH2 MLH1 NTRK3 ROS1
BRAF ERBB2* FOXL2 JAK2 MPL PDGFRA SMAD4
CCNE1 ERBB4 GNA11 JAK3 MYC* PIK3CA SMARCB1
CDH1 EZH2 GNAQ KDR NOTCH1 PTEN SMO

CNVs detected and verified by NIST reference standards are indicated by *
CNVs can also be detected in genes indicated by

Panel specifications

Enrichment chemistry Multiplex PCR using tiled amplicons
Number of pools 1 pool
Number of genes/amplicons 60/341
Number of targets Hotspots in 60 genes; CNVs for 14 genes
Variant types SNVs, small and medium indels, and CNVs
Average amplicon size 125bp (range 86bp – 185bp)
Recommended DNA input range 5ng to 80ng
Sample types DNA from tissue or blood; cfDNA; FFPE
Mapping rate 99.3% ± 0.3%
% on-target aligned reads 99.5% ± 0.1 %
Coverage uniformity (% targets with >0.2X mean coverage) 98.2% ± 0.7%
Total assay time (from DNA to sequencer) <8 hours
Sequencing platforms Illumina®, MGISEQ™ and Ion Torrent™ platforms

See the data

Effective CNV calling

The ONCO/Reveal Multi-Cancer v4 with CNV Panel for MGI detects copy number variations in 14 genes, including ERBB2.

Normalized gene count or DISH HER2/chr17 Ratios

Pillar technology

Pillar provides a seamlessly integrated NGS workflow, from panel design to data analysis.

ampPD™

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Primer design platform for reliable amplicon specificity

SLIMamp®

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Enrichment chemistry that enables single-tube amplification

PiVAT®

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Bioinformatic tools that deliver sensitive, robust variant calls

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