Pillar® ONCO/Reveal™ Multi-Cancer RNA Fusion v2 Panel

The ONCO/Reveal Multi-Cancer RNA Fusion v2 Panel is a robust NGS assay that interrogates multiple gene rearrangement regions of interest across solid tumor samples for cancers including but not limited to NSCLC, thyroid, and prostate cancer. The assay uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology, a tiled amplicon-based library prep chemistry for efficient single-tube target enrichment.

Panel highlights

Targets include NSCLC, thyroid, and prostate cancers

18 driver genes and >80 partner genes, plus MET 14 exon skipping

Specifically designed to detect RNA fusions

Compatible with RNA from tissue or FFPE

For research use only. Not for use in diagnostic procedures.

Panel targets

Target driver genes Fusion partners
ALK EML4, CLTC, HIP1, KIF5B, KLC1, STRN, TFG, TPM3, TPR, MSN
RET CCDC6, CUX1, KIF5B, NCOA4, TRIM33, PRKARA1, NCOA4
ROS1 CCDC6, CD74, CLTC, EZR, GOPC, LRIG3, MSN, SDC4, SLC34A2, TFG, TPM3
NRG1 CD74, SLC3A2, VAMP2
PBX1 TCF3
FGFR3 TACC3, BAIAP2L1
FGFR2 BICC1, CASP7
EGFR SPET14, PSPH, RAD51, EGFRVIII
NTRK1 TPM3, TFG, LMNA, SQSTM1, CHTOP, ARHGEF2, NFASC, IRF2BP2, PPL, BCAN, SCYL3, TP53, CD74, MPRIP, TPR
NTRK2 AFAP1, NACC2, BCR, TRIM24, QKI, PAN3, SQSTM1
NTRK3 ETV6, BTBD1, EML4, SQSTM1, TFG, RBPMS
BRAF KIAA1549, MKRN1, FAM131B, AKAP9
RAF1 ESRP1, SRGAP3
MET KIF5B, Ex14 skipping
PPARG PAX8, CREB3L2
ERG TMPRSS2
PRKACA DNAJB1
TFE3 SFPQ, ASPSCR1, CLTC, PRCC, NONO
3′/5′ Expression Imbalance Ratio Assessed
ALK ROS1 RET NTRK1 NTRK2 NTRK3 FGFR3 NRG1 PBX1
Expression Control Genes
HMBS TBP

Panel specifications

Enrichment chemistry Multiplex PCR using tiled amplicons
Number of pools 1 pool
Number of genes/partners 18 genes and >80 partners, plus MET 14 exon skipping
Variant types Fusion RNA transcripts
Average amplicon size 120bp
Input RNA 10ng – 50ng
Sample types RNA from FFPE or tissue
Total assay time (from DNA to sequencer) <9-10 hours
Sequencing platforms Illumina®, MGISEQ™ and Ion Torrent™

See the data

Robust fusion detection

The ONCO/Reveal Multi-Cancer RNA Fusion v2 Panel accurately detects fusions across various reference standards.

SeraSeq® Fusion v3
Fusion partners Detected?
EML4-ALK Yes
KIF5B-RET Yes
NCOA4-RET Yes
CD74-ROS1 Yes
SLC34A-ROS1 Yes
TPM3-NTRK1 Yes
FGFR3-BAIAP2L1 Yes
PAX8-PPARG1 Yes
FGFR3-TACC3 Yes
ETV6-NTRK3 Yes
LMNA-NTRK1 Yes
TMPRSS2-ERG Yes
EGFR-SEPT14 Yes
MET Exon 14 skipping Yes
EGFR variant III Yes
SeraSeq NTRK FFPE
Fusion partners Detected?
TPM3-NTRK1 Yes
LMNA-NTRK1 Yes
IRF2BP2-NTRK1 Yes
SQSTM1-NTRK1 Yes
TFG-NTRK1 Yes
AFAP1-NTRK2 Yes
NACC2-NTRK2 Yes
QKI-NTRK2 Yes
TRIM24-NTRK2 Yes
PAN3-NTRK2 Yes
ETV6-NTRK3 Yes
BTBD1-NTRK3 Yes
Horizon™ 5 Fusion
Fusion partners Detected?
EML4-ALK Yes
CCDC6-RET Yes
SLC34A-ROS1 Yes
TPM3-NTRK1 Yes
ETV6-NTRK3 Yes

Pillar technology

Pillar provides a seamlessly integrated NGS workflow, from panel design to data analysis.

ampPD™

icon of ampPD primer as located on DNA

Primer design platform for reliable amplicon specificity

SLIMamp®

test tube icon

Enrichment chemistry that enables single-tube amplification

PiVAT®

PiVat icon

Bioinformatic tools that deliver sensitive, robust variant calls

Contact an expert

Contact us for technical information or to get a quote

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