Pillar® ONCO/Reveal™ Multi-Cancer Panel

The ONCO/Reveal Multi-Cancer Panel is a robust NGS assay that interrogates 56 genes of interest across multiple solid tumor cancer types. The assay uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology, a tiled amplicon-based library prep chemistry for efficient single-tube target enrichment.

Panel highlights

Requires as little as 2.5 ng of input DNA

Targets >7,200 hotspots in multiple solid tumor types 

Detection of 1% allele frequency in somatic cancers

From sample to library in as little as 5 hours (2 hrs hands on) 

For research use only. Not for use in diagnostic procedures.

Panel targets

ABL1 CSF1R FGFR1 HNF1A KRAS NTRK1 ROS1
AKT1 CTNNB1 FGFR2 HRAS MAP2K1 PDGFRA SMAD4
ALK DDR2 FGFR3 IDH1 MET PIK3CA SMARCB1
APC EGFR FLT3 IDH2 MLH1 PTEN SMO
ATM ERBB2 FOXL2 JAK2 MPL PTPN11 SRC
BRAF ERBB4 GNA11 JAK3 NOTCH1 RAC1 STK11
CDH1 EZH2 GNAQ KDR NPM1 RB1 TP53
CDKN2A FBXW7 GNAS KIT NRAS RET VHL

Panel specifications

Enrichment chemistry Multiplex PCR using tiled amplicons
Number of pools 1 pool
Number of genes/amplicons 56/251
Number of targets >7,200 hotspots, 6,000 SNVs, 1,200 indels, 24kb total size
Variant types SNVs, indels
Average amplicon size 143bp
Recommended DNA input range 5ng to 80ng
Sample types DNA from tissue or blood; cfDNA; FFPE
Mapping rate 99.8% ± 0.0%
% on-target aligned reads 96.2% ± 0.1%
Coverage uniformity (% targets with >0.2X mean coverage) 96.1 ± 0.1%
Total assay time (from DNA to sequencer) <8 hours
Sequencing platforms Illumina® and MGISEQ™

See the data

Single-vial Amplification Based NGS with Rapid Turn-Around-Time for Interrogation of Variants in Tumors

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Clinical and biomarker results from Phase I-II study of PI3K inhibitor BYL719 (Apelisib) plus Nab-paclitaxel in HER2-negative Metastatic Breast Cancer

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Evaluation of the Pillar NGS SLIMap Cancer Hotspot Panel

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Sensitive variant detection

The ONCO/Reveal Multi-Cancer Panel demonstrated high sensitivity and concordance across the range of allele frequencies.
Plot showing multi-cancer concordance
Variant # samples VAF range (%)
BRAF V600E 4 1.6 - 44
BRAF Other 2 5
EGFR exon19 deletion 7 1.4 - 42
EGFR exon 20 insertion 2 18 - 22
EGFR L858R 4 16 - 80
EGFR T790M 2 3 - 10
KRAS G12A/C/D/F/S/V 15 6 - 33
KRAS G12R 1 28
KRAS K117 1 16
KIT exon11 deletion 2 35, 63
MET exon14 skipping 2 72, 75
NRAS Q61K/R 2 40, 80
NRAS G12C 1 38
PIK3CA E545K/H1047R 2 6, 36
STK11 missense 2 35, 28
STK11 deletion 1 20
TP53 nonsense 5 21

Concordance demonstrated with low DNA inputs

concordance for low inputs

Pillar technology

Pillar provides a seamlessly integrated NGS workflow, from panel design to data analysis.

ampPD™

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Primer design platform for reliable amplicon specificity

SLIMamp®

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Enrichment chemistry that enables single-tube amplification

PiVAT®

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Bioinformatic tools that deliver sensitive, robust variant calls

Contact an expert

Contact us for technical information or to get a quote

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