Pillar® ONCO/Reveal™ Multi-Cancer RNA Fusion v2 Panel

The ONCO/Reveal Multi-Cancer with CNV and RNA Fusion Panel is a combined DNA/RNA multi-cancer panel. The assay combines the DNA-based ONCO/Reveal Multi-Cancer v4 with CNV Panel with the ONCO/Reveal Multi-Cancer RNA Fusion v2 Panel allowing for joint sequencing. The assay uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology, a tiled amplicon-based library prep chemistry for efficient single-tube target enrichment.

Panel highlights

Multiple solid tumor types including NSCLC, thyroid, and prostate 

SNVs, indels, CNVs, and RNA fusions in one product

60 hotspots and 11 CNVs 

Fusions in 18 driver genes plus expression of 14 partners

For research use only. Not for use in diagnostic procedures.

Variants and CNVs detected from DNA

ABL1 CDKN2A FBXW7 GNAS KIT NPM1 PTPN11 SRC
AKT1 CSF1R FGFR1 HNF1A KRAS NRAS RAC1 STK11
ALK CTNNB1 FGFR2 HRAS MAP2K1 NTRK1 RB1 TP53
APC DDR2 FGFR3 IDH1 MET* NTRK2 RET VHL
ATM EGFR* FLT3 IDH2 MLH1 NTRK3 ROS1
BRAF ERBB2* FOXL2 JAK2 MPL PDGFRA SMAD4
CCNE1 ERBB4 GNA11 JAK3 MYC* PIK3CA SMARCB1
CDH1 EZH2 GNAQ KDR NOTCH1 PTEN SMO

CNVs detected and verified by NIST reference standards are indicated by *
CNVs can also be detected in genes indicated by

Fusions and expression insights detected from RNA

Driver gene fusions (fusion partners not listed)
ALK EGFR FGFR2 MET NTRK1 NTRK3 PPARG RAF ROS1
BRAF ERG FGFR3 NRG1 NTRK2 PBX1 PRKACA RET TFE3
Expression imbalance
ALK FGFR3 NRG1 NTRK1 NTRK2 NTRK3 PBX1 RET ROS1
Expression control
HMBS TMP

Panel specifications

ONCO/Reveal Multi-Cancer v4 with CNV ONCO/Reveal Multi-Cancer RNA Fusion v2
Enrichment chemistry Multiplex PCR using tiled amplicons Multiplex PCR using tiled amplicons
Number of pools 2 pools 2 pools
Number of genes/amplicons 60/341 18/>80 plus MET 14 exon skipping
Number of targets Hotspots in 60 genes; CNVs for 11 genes Fusions in 18 driver genes; expression for 11 genes
Variant types SNVs, small and medium indels, and CNVs Fusion RNA transcripts
Average amplicon size 125bp (range 86bp – 185bp) 120bp
Recommended input range 5ng to 80ng DNA 10ng to 50ng RNA
Sample types DNA from tissue or blood; FFPE RNA from FFPE or tissue
Mapping rate 99.3% ± 0.3% n/a
% on-target aligned reads 99.5% ± 0.1 % n/a
Coverage uniformity (% targets with >0.2X mean coverage) 98.2% ± 0.7% n/a
Total assay time (from DNA to sequencer) <8 hours <9-10 hours
Sequencing platforms Illumina®, MGISEQ™ and Ion Torrent™ platforms Illumina, MGISEQ and Ion Torrent platforms

Pillar technology

Pillar provides a seamlessly integrated NGS workflow, from panel design to data analysis.

ampPD™

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Primer design platform for reliable amplicon specificity

SLIMamp®

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Enrichment chemistry that enables single-tube amplification

PiVAT®

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Bioinformatic tools that deliver sensitive, robust variant calls

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