Pillar® ONCO/Reveal™ Lung and Colon Cancer Panel

The ONCO/Reveal Lung and Colon Cancer Panel is a robust NGS assay that interrogates 22 genes of interest across lung and colon cancer samples. The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology, a tiled amplicon-based library preparation chemistry for efficient single-tube target enrichment.

Panel highlights

Requires as little as 2.5ng of input DNA

Targets more than 1,800 hotspots

From sample to library in as little as 5 hours (2hrs hands on)

Robust to FFPE degradation

For research use only. Not for use in diagnostic procedures.

Panel targets

AKT1 EGFR FGFR2 NOTCH1 STK11
ALK ERBB2 FGFR3 NRAS TP53
BRAF ERBB4 KRAS PIK3CA
CTNNB1 FBXW7 MAP2K1 PTEN
DDR2 FGFR1 MET SMAD4

Panel specifications

Enrichment chemistry Multiplex PCR using tiled amplicons
Number of pools 1 pool
Number of genes/amplicons 22/103
Number of targets >1,800 hotspots, 11kb total size
Variant types SNVs, indels
Average amplicon size 147bp
Recommended DNA input range 2.5ng to 80ng
Sample types DNA from tissue or blood; cfDNA; FFPE
Mapping rate 97.4% ± 2.5%
% on-target aligned reads 96.8% ± 1.3%
Coverage uniformity (% targets with >0.2X mean coverage) 99.8% ± 0.7%
Total assay time (from DNA to sequencer) <8 hours
Sequencing platforms Illumina® and MGISEQ™; assay is coming soon for the Ion Torrent™ platform

See the data

slimAMP concordance data using Pillar ONCO/Reveal Lung and Colon Panel

Sensitive variant detection

The ONCO/Reveal Lung and Colon Cancer Panel is highly precise even with low DNA inputs, demonstrating high concordance (R2 >99%) between input amounts across a wide range of variant allele frequencies. Assays and analyses were performed at Dartmouth Hitchcock Medical Center and presented at AMP 2016.

Robust variant detection

The ONCO/Reveal Lung and Colon Cancer Panel is accurate, precise, repeatable and sensitive to near the limit of detection regardless of FFPE quality. In the figure to the right, formalin-compromised reference standards (Horizon Discovery) were evaluated using the ONCO/Reveal Lung and Colon Cancer Panel, which demonstrated accurate results from degraded samples. N=10; error bars=standard deviation

Pillar technology

Pillar provides a seamlessly integrated NGS workflow, from panel design to data analysis.

ampPD™

icon of ampPD primer as located on DNA

Primer design platform for reliable amplicon specificity

SLIMamp®

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Enrichment chemistry that enables single-tube amplification

PiVAT®

PiVat icon

Bioinformatic tools that deliver sensitive, robust variant calls

Contact an expert

Contact us for technical information or to get a quote

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