Pillar® ONCO/Reveal™ PillarHS Lung Panel

The ONCO/Reveal PillarHS Lung Panel is a robust NGS assay that interrogates 17 lung cancer genes of interest. The panel includes Unique molecular IDs (UIDs) to increase sensitivity and is specifically designed for circulating cell-free tumor DNA (ccfDNA/cfDNA/ctDNA, liquid biopsies). The assay uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology, a tiled amplicon-based library prep chemistry for efficient single-tube target enrichment.

Panel highlights

Targets known lung cancer-related genes 

Specifically designed for cell-free DNA

Achieve variant detection at <1% VAF with UIDs 

>99% coverage uniformity at 0.2x mean

For research use only. Not for use in diagnostic procedures.

Panel targets

AKT1 DDR2 ERBB4 KRAS NRAS STK11
ALK EGFR FGFR1 MAP2K1 PIK3CA TP53
BRAF ERBB2 FGFR3 MET PTEN

Panel specifications

Enrichment chemistry Multiplex PCR using tiled amplicons
Number of pools 1 pool
Number of genes/amplicons 17/81
Total panel size 8kb
Variant types SNVs, indels
Average amplicon size 147bp
Recommended DNA input range 15ng to 50ng
Sample type ccfDNA/cfDNA/ctDNA
Sensitivity <1% VAF
% on-target aligned reads 93.2% ± 2.9%
Coverage uniformity (% targets with >0.2X mean coverage) 99.8% ± 0.7%
Total assay time (from DNA to sequencer) <9-10 hours
Sequencing platforms Illumina® and MGISEQ™

See the data

Sensitive variant detection

Cell-free reference DNA (Horizon Discovery) was tested at a nominal level of 0.1% and 0.25%. The ONCO/Reveal pillarHS Lung Panel demonstrated high sensitivity down to 0.1% VAF.


Input DNA was comparable to 20ng of actual cell free DNA.

Gene Variant Exon 0.1% (nominal) 0.25% (nominal) Wild type
EGFR Δ E746-A750 19 0.11% 0.34% 0.0%
EGFR V769-D770insASV 20 0.28% 0.23% 0.0%
EGFR T790M 20 0.12% 0.23% 0.0%
EGFR L858R 21 0.15% 0.19% 0.0%
KRAS G12D 2 0.20% 0.10% 0.0%
NRAS A59T 3 0.09% 0.41% 0.0%
NRAS Q61K 3 0.09% 0.50% 0.0%
PIK3CA E545K 10 0.22% 0.67% 0.0%

Pillar technology

Pillar provides a seamlessly integrated NGS workflow, from panel design to data analysis.

ampPD™

icon of ampPD primer as located on DNA

Primer design platform for reliable amplicon specificity

SLIMamp®

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Enrichment chemistry that enables single-tube amplification

PiVAT®

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Bioinformatic tools that deliver sensitive, robust variant calls

Contact an expert

Contact us for technical information or to get a quote

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