Pillar® ONCO/Reveal™ Essential MPN Panel
The ONCO/Reveal Essential MPN Panel is a robust assay that simultaneously tests for key
mutations within the MPL, JAK2, and CALR genes. Up to 75 samples can be multiplexed on a
single MiSeq v2 Nano cartridge. The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology for efficient single-tube target enrichment.
Panel highlights
Targets key myeloprolifitive neoplasm genes
Two exons in JAK2, one each in MPL and CALR
From sample to library in as little as 5 hours (2hrs hands on)
High throughput
For research use only. Not for use in diagnostic procedures.
Panel targets
| Gene (exon #) |
|---|
| MPL (ex10) |
| JAK2 (ex12) |
| JAK2 (ex14) |
| CALR (ex9) |
Panel specifications
| Enrichment chemistry | Multiplex PCR using tiled amplicons |
| Number of pools | 1 pool |
| Number of genes/amplicons | 3/7 |
| Number of targets | MPL ex10, JAK2 ex12, JAK2 ex14, CALR ex9 |
| Variant types | SNVs, small and medium indels |
| Average amplicon size | 167bp (range 110bp – 190bp) |
| Recommended DNA input range | 10ng to 80ng (20ng recommended) |
| Sample types | Whole blood, cfDNA, PBMCs |
| Mapping rate | 99.8% ± 0.1% |
| % on-target aligned reads | 99.9% ± 0.0% |
| Coverage uniformity (% targets with >0.2X mean coverage) | 100% |
| Total assay time (from DNA to sequencer) | <8 hours |
| Sequencing platforms | Illumina®, MGISEQ™, and Ion Torrent™ platforms |
See the data
| Gene (exon #) | Variant | SeraCare VAF (%) | RepID | VAF from serial dilution series (%) | ||
|---|---|---|---|---|---|---|
| 1:2 | 1:4 | 1:8 | ||||
| MPL (ex10) | c.1544G>T | 7.17 | Rep1 | 3.91 | 2.52 | 1.15 |
| MPL (ex10) | c.1544G>T | 7.17 | Rep2 | 4.26 | 2.05 | 1.05 |
| MPL (ex10) | c.1544G>T | 7.17 | Rep3 | 3.78 | 2.86 | 1.52 |
| JAK2 (ex12) | c.1624_1629del | 15.74 | Rep1 | 5.52 | 2.55 | 1.09 |
| JAK2 (ex12) | c.1624_1629del | 15.74 | Rep2 | 5.78 | 2.2 | 1.08 |
| JAK2 (ex12) | c.1624_1629del | 15.74 | Rep3 | 5.95 | 2.05 | 1.71 |
| JAK2 (ex14) | p.Val617Phe | 7.8 | Rep1 | 2.88 | 1.25 | 0.69 |
| JAK2 (ex14) | p.Val617Phe | 7.8 | Rep2 | 3.39 | 1.26 | 0.56 |
| JAK2 (ex14) | p.Val617Phe | 7.8 | Rep3 | 2.97 | 1.08 | 0.65 |
| CALR (ex9) | 52 bp deletion | 7.28 | Rep1 | 3.52 | 1.71 | 1.57 |
| CALR (ex9) | 52 bp deletion | 7.28 | Rep2 | 3.54 | 1.52 | 1.37 |
| CALR (ex9) | 52 bp deletion | 7.28 | Rep3 | 3.31 | 1.82 | 1.23 |
