Pillar® ONCO/Reveal™ Essential MPN Panel

The ONCO/Reveal Essential MPN Panel is a robust assay that simultaneously tests for key
mutations within the MPL, JAK2, and CALR genes. Up to 75 samples can be multiplexed on a
single MiSeq v2 Nano cartridge. The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology for efficient single-tube target enrichment.

Panel highlights

Targets key myeloprolifitive neoplasm genes 

 Two exons in JAK2, one each in MPL and CALR

From sample to library in as little as 5 hours (2hrs hands on) 

High throughput

For research use only. Not for use in diagnostic procedures.

Panel targets

Gene (exon #)
MPL (ex10)
JAK2 (ex12)
JAK2 (ex14)
CALR (ex9)

Panel specifications

Enrichment chemistry Multiplex PCR using tiled amplicons
Number of pools 1 pool
Number of genes/amplicons 3/7
Number of targets MPL ex10, JAK2 ex12, JAK2 ex14, CALR ex9
Variant types SNVs, small and medium indels
Average amplicon size 167bp (range 110bp – 190bp)
Recommended DNA input range 10ng to 80ng (20ng recommended)
Sample types Whole blood, cfDNA, PBMCs
Mapping rate 99.8% ± 0.1%
% on-target aligned reads 99.9% ± 0.0%
Coverage uniformity (% targets with >0.2X mean coverage) 100%
Total assay time (from DNA to sequencer) <8 hours
Sequencing platforms Illumina®, MGISEQ™, and Ion Torrent™ platforms

See the data

Gene (exon #) Variant SeraCare VAF (%) RepID VAF from serial dilution series (%)
1:2 1:4 1:8
MPL (ex10) c.1544G>T 7.17 Rep1 3.91 2.52 1.15
MPL (ex10) c.1544G>T 7.17 Rep2 4.26 2.05 1.05
MPL (ex10) c.1544G>T 7.17 Rep3 3.78 2.86 1.52
JAK2 (ex12) c.1624_1629del 15.74 Rep1 5.52 2.55 1.09
JAK2 (ex12) c.1624_1629del 15.74 Rep2 5.78 2.2 1.08
JAK2 (ex12) c.1624_1629del 15.74 Rep3 5.95 2.05 1.71
JAK2 (ex14) p.Val617Phe 7.8 Rep1 2.88 1.25 0.69
JAK2 (ex14) p.Val617Phe 7.8 Rep2 3.39 1.26 0.56
JAK2 (ex14) p.Val617Phe 7.8 Rep3 2.97 1.08 0.65
CALR (ex9) 52 bp deletion 7.28 Rep1 3.52 1.71 1.57
CALR (ex9) 52 bp deletion 7.28 Rep2 3.54 1.52 1.37
CALR (ex9) 52 bp deletion 7.28 Rep3 3.31 1.82 1.23

Pillar technology

Pillar provides a seamlessly integrated NGS workflow, from panel design to data analysis.

ampPD™

icon of ampPD primer as located on DNA

Primer design platform for reliable amplicon specificity

SLIMamp®

test tube icon

Enrichment chemistry that enables single-tube amplification

PiVAT®

PiVat icon

Bioinformatic tools that deliver sensitive, robust variant calls

Contact an expert

Contact us for technical information or to get a quote

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