How do I hit all my targets with one panel?
Pillar® ONCO/Reveal™ Custom Panels
We get it. You have a unique set of gene targets, but you don’t have time to develop your own NGS assay. Let our team of scientists develop a custom panel to interrogate your regions of interest. You provide the targets (or select from a list), and we’ll deliver a custom panel in as little as 6-8 weeks.
Like all Pillar assays, your custom panel will be designed using the ampPD™ intelligent primer design platform to enable single-tube amplification via our proprietary SLIMamp® technology. Your custom panel also comes with access to the PiVAT® bioinformatics pipeline, which enables detection down to 1% VAF (<1% with UIDs).
Detect fusions, CNVs, SNVs, and indels
As little as 1ng input DNA
Fully optimized panels in as little as 6-8 weeks
For research use only. Not for use in diagnostic procedures.
See our custom panels in action
|Disease states||Oncology, genetic diseases, ophthalmology, familial hypercholesterolemia, thalassemia, metabolism, carrier screening, NIPT, PGx, IBD, and more|
|Sample types||Solid tumors, liquid biopsy, myeloid, germline, FFPE, blood, plasma, serum, other biofluids|
|Variant types||Fusions, CNV, SNV, indels|
|Coverage||Hotspots or full gene CDS (overlapping amplicons) from single-tube amplification|
|PiVAT||Cloud or local installation|
|Support||Field application scientist team|
|Enrichment chemistry||Multiplex PCR using tiled amplicons|
|Number of pools||1 pool|
|Number of amplicons||Up to 5,000|
|Variant types||SNVs, indels, fusions, CNVs|
|Limit of detection (LoD)||1%-2% VAF without UIDs (10ng input DNA) or <1% VAF with UIDs (20ng input DNA)|
|Input DNA range||1ng to 80ng (10ng recommended)|
|Sample types||Genomic DNA, Cell-free DNA, FFPE DNA, fresh frozen tissue, RNA|
|% on-target aligned reads*||>90%|
|Coverage uniformity (% targets with >0.2X mean coverage)*||>90%|
|Total assay time (from sample to sequencer)||<8 hours|
|Interactive design and functional testing||Yes|
|Designer; Analysis software||ampPD; PiVAT|
|Sequencing platforms||Illumina®, MGISEQ™, and Ion Torrent™|
|Configurable kit contents||Primer pool, indexing primers, UDG, beads|
See the data
Sensitive and robust variant detection
ONCO/Reveal assays are accurate, precise, repeatable and sensitive to near the limit of detection regardless of FFPE quality. In the figure above, formalin-compromised reference standards (Horizon Discovery) were evaluated using the ONCO/Reveal Lung and Colon Cancer Panel, which demonstrated accurate results from degraded samples.
N=10; error bars=standard deviation
Design and delivery timelines
Pillar provides a seamlessly integrated NGS workflow, from panel design to data analysis.
Primer design platform for reliable amplicon specificity
Enrichment chemistry that enables single-tube amplification
Bioinformatic tools that deliver sensitive, robust variant calls