Pillar® ONCO/Reveal™ BRCA1 & BRCA2 Somatic Panel
The ONCO/Reveal BRCA1 & BRCA2 Somatic Panel is specifically designed with shorter amplicons to detect mutations in FFPE samples. The assay sequences the entire coding region of the BRCA1 and BRCA2 genes plus 10bp of flanking introns. Pillar also offers a panel optimized for germline BRCA1 & BRCA2 mutation detection. Both panels use
proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology for
efficient single-tube target enrichment.
Panel highlights
Full BRCA1 and BRCA2 CDS plus flanking sequences
From sample to library in as little as 5 hours (2hrs hands on)
Designed to detect mutations in FFPE samples
>99% coverage uniformity at 0.2x mean coverage
For research use only. Not for use in diagnostic procedures.
Panel specifications
| Enrichment chemistry | Multiplex PCR using tiled amplicons |
| Number of pools | 1 pool |
| Number of genes/amplicons | 2/196 |
| Number of targets | Full coding gene sequence (CDS) with flanking intronic regions (10bp) |
| Variant types | SNVs, indels |
| Average amplicon size | 112bp |
| Input DNA range | 10ng to 60ng (20ng recommended) |
| Sample types | FFPE |
| Mapping rate | 98.4% ± 1.0% |
| % on-target aligned reads | 99.9% ± 0.0% |
| Coverage uniformity (% targets with >0.2X mean coverage) | 99.6% ± 0.5% |
| Total assay time (from DNA to sequencer) | <8 hours |
| Sequencing platforms | Illumina®, MGISEQ™, and Ion Torrent™ |
