Pillar® ONCO/Reveal™ BRCA1 & BRCA2 Somatic Panel

The ONCO/Reveal BRCA1 & BRCA2 Somatic Panel is specifically designed with shorter amplicons to detect mutations in FFPE samples. The assay sequences the entire coding region of the BRCA1 and BRCA2 genes plus 10bp of flanking introns. Pillar also offers a panel optimized for germline BRCA1 & BRCA2 mutation detection. Both panels use proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology for efficient single-tube target enrichment.

Panel highlights

Full BRCA1 and BRCA2 CDS plus flanking sequences

From sample to library in as little as 5 hours (2hrs hands on)

Designed to detect mutations in FFPE samples

>99% coverage uniformity at 0.2x mean coverage

For research use only. Not for use in diagnostic procedures.

Panel specifications

Enrichment chemistry	Multiplex PCR using tiled amplicons
Number of pools	1 pool
Number of genes/amplicons	2/196
Number of targets	Full coding gene sequence (CDS) with flanking intronic regions (10bp)
Variant types	SNVs, indels
Average amplicon size	112bp
Input DNA range	10ng to 60ng (20ng recommended)
Sample types	FFPE
Mapping rate	98.4% ± 1.0%
% on-target aligned reads	99.9% ± 0.0%
Coverage uniformity (% targets with >0.2X mean coverage)	99.6% ± 0.5%
Total assay time (from DNA to sequencer)	<8 hours
Sequencing platforms	Illumina®, MGISEQ™ and Ion Torrent™

Pillar technology

Pillar provides a seamlessly integrated NGS workflow, from panel design to data analysis.

ampPD™

Primer design platform for reliable amplicon specificity

SLIMamp®

Enrichment chemistry that enables single-tube amplification

PiVAT®

Bioinformatic tools that deliver sensitive, robust variant calls