Pillar® ONCO/Reveal™ BRCA1 & BRCA2 Panel

The ONCO/Reveal BRCA1 & BRCA2 Panel is a robust NGS assay to sequence the entire coding regions of the BRCA1 and BRCA2 genes plus 20bp of flanking introns. The assay uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology, a tiled amplicon-based library prep chemistry for efficient single-tube target enrichment.

Panel highlights

Full BRCA1 and BRCA2 CDS plus flanking sequences

As little as 5ng input DNA
 

From sample to library in as little as 5 hours (2hrs hands on)

up to >98% coverage uniformity at 0.2x mean coverage

For research use only. Not for use in diagnostic procedures.

Panel specifications

Enrichment chemistry Multiplex PCR using tiled amplicons
Number of pools 1 pool
Number of genes/amplicons 2/91
Number of targets Full coding gene sequence (CDS) with flanking intronic regions (20bp), 17 kb total size
Variant types SNVs, indels
Average amplicon size 331bp
Recommended DNA input range 5ng to 100ng
Sample types Genomic DNA from blood or tissue; cfDNA
Mapping rate 99.8% ± 0.3%
% on-target aligned reads 99.8% ± 0.2%
Coverage uniformity (% targets with >0.2X mean coverage) 98.3% ± 1.3%
Total assay time (from DNA to sequencer) <8 hours
Sequencing platforms Illumina®

See the data

BRCA coverage uniformaty histogram

Excellent coverage uniformity and high predictive agreement

The ONCO/Reveal BRCA1 & BRCA2 Panel demonstrated excellent coverage uniformity across 39 cell lines and reference standards, as shown in the graph below. The panel was also reported to show high positive predictive agreement (PPA) and negative predictive agreement (NPA)*.
*Schenk D, Song G, Ke Y, Wang Z (2017) Amplification of overlapping DNA amplicons in a single-tube multiplex PCR for targeted next-generation sequencing of BRCA1 and BRCA2. PLoS ONE 12(7): e0181062. https://doi.org/10.1371/journal.pone.0181062

Pillar technology

Pillar provides a seamlessly integrated NGS workflow, from panel design to data analysis.

ampPD™

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Primer design platform for reliable amplicon specificity

SLIMamp®

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Enrichment chemistry that enables single-tube amplification

PiVAT®

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Bioinformatic tools that deliver sensitive, robust variant calls

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Contact us for technical information or to get a quote

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