Pillar® INHERIT/Reveal™ CFTR Panel

The INHERIT/Reveal CFTR Panel covers the entire CDS of the cystic fibrosis transmembrane conductance regulator (CFTR) gene and can multiplex up to 384 samples on a MiSeq with 2×250 PE reads. The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology for efficient single-tube target enrichment.

Panel highlights

Full CFTR CDS plus flanking introns 

Captures large deletions as well as SNVs

As little as 5ng input DNA 

High-throughput 

For research use only. Not for use in diagnostic procedures.

Panel specifications

Enrichment chemistry Multiplex PCR using tiled amplicons
Number of pools 1 pool
Number of genes/amplicons 1/34
Number of targets Full CDS + 10bp flanking introns and intron 12/22
Variant types SNVs, indels, 3 large deletions (10kb - 21kb)
Average amplicon size 361bp (324bp - 405bp range)
Input DNA range 5ng to 500ng
Sample types Genomic DNA
Mapping rate 99.8% ± 0.2%
% on-target aligned reads 99.9% ± 0.1%
Coverage uniformity (% targets with >0.2X mean coverage) 100%
Total assay time (from DNA to sequencer) <8 hours
Sequencing platforms Illumina®

See the data

Evaluation of a high-throughput NGS SLIMamp CFTR Assay

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Pillar technology

Pillar provides a seamlessly integrated NGS workflow, from panel design to data analysis.

ampPD™

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Primer design platform for reliable amplicon specificity

SLIMamp®

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Enrichment chemistry that enables single-tube amplification

PiVAT®

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Bioinformatic tools that deliver sensitive, robust variant calls

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Contact us for technical information or to get a quote

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