Pillar® INHERIT/Reveal™ CFTR Panel

The INHERIT/Reveal CFTR Panel covers the entire CDS of the cystic fibrosis transmembrane conductance regulator (CFTR) gene and can multiplex up to 384 samples on a MiSeq with 2×250 PE reads. The panel uses proprietary Stem-Loop Inhibition-Mediated amplification (SLIMamp®) technology for efficient single-tube target enrichment.

Panel highlights

Full CFTR CDS plus flanking introns 

Captures large deletions as well as SNVs

As little as 5ng input DNA 


For research use only. Not for use in diagnostic procedures.

Panel specifications

Enrichment chemistry Multiplex PCR using tiled amplicons
Number of pools 1 pool
Number of genes/amplicons 1/34
Number of targets Full CDS + 10bp flanking introns and intron 12/22
Variant types SNVs, indels, 3 large deletions (10kb - 21kb)
Average amplicon size 361bp (324bp - 405bp range)
Input DNA range 5ng to 500ng
Sample types Genomic DNA
Mapping rate 99.8% ± 0.2%
% on-target aligned reads 99.9% ± 0.1%
Coverage uniformity (% targets with >0.2X mean coverage) 100%
Total assay time (from DNA to sequencer) <8 hours
Sequencing platforms Illumina®

See the data

Evaluation of a high-throughput NGS SLIMamp CFTR Assay


Pillar technology

Pillar provides a seamlessly integrated NGS workflow, from panel design to data analysis.


icon of ampPD primer as located on DNA

Primer design platform for reliable amplicon specificity


test tube icon

Enrichment chemistry that enables single-tube amplification


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Bioinformatic tools that deliver sensitive, robust variant calls

Contact an expert

Contact us for technical information or to get a quote

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