BRCA 1/2 Germline Mutation

The Pillar Biosciences SLIMamp Hereditary Cancer Panel: BRCA1/2 is an accurate, fast solution for sequencing the entire BRCA1 and BRCA2 genes. Every base in the coding exons is 100% covered, plus 20bp flanking regions. The assay calls all SNVs and small and medium indels. Multiplex your samples coming from whole blood for up to 96 samples per run.

Benefits:

  • Automatable single tube prep
  • Total time is 6 hours
  • Under 2 hours hands-on time
  • Greater than 99.5% assay sensitivity and specificity
BRCA Panel Statistics
Sample type gDNA
Amplicon # 91
On target rate 99.9% ± 0.2%
Coverage uniformity %>0.20 × Mean Coverage: 98.3% ± 1.3%
%>0.10 × Mean Coverage: 100.0% ± 0.0%
Variant frequency 1-2%

Genome Browser BRCA

Concatenated IGV rendering of targeted regions of BRCA2 gene with an example of relative sequencing depths.