Pillar® ONCO/Reveal™ BRCA1 & BRCA2 plus CNV Panel

The ONCO/Reveal BRCA1 & BRCA2 plus CNV Panel is designed for the detection of exon-level copy number variation (CNV) across the entire coding region of the BRCA1 and BRCA2 genes (as well as 10bp flanking introns). The assay is designed with shorter amplicons to allow for detection of mutations from FFPE samples, while also being compatible with intact genomic DNA from blood samples.

Panel highlights

Full BRCA1 and BRCA2 CDS plus flanking sequences

Detects exon level CNVs, SNVs, and indels

Detection down to 1% VAF even with low sample input/quality

Robust to FFPE degradation

For research use only. Not for use in diagnostic procedures.

Panel specifications

Enrichment chemistry	Multiplex PCR using tiled amplicons
Number of pools	1 pool
Number of genes/amplicons	2/283
Number of targets	Full coding gene sequence (CDS) with flanking intronic regions (10bp)
Variant types	Exon level CNV, SNV, small and medium indels
Average amplicon size	155 bp
Recommended DNA input range	20 ng to 80 ng
Sample types	DNA from tissue or blood; FFPE
Mapping rate	99.0% ± 0.3%
% on-target aligned reads	98.6% ± 0.2%
Coverage uniformity (% targets with >0.2X mean coverage)	98.0% ± 0.1%
Total assay time (from DNA to sequencer)	<8 hours
Sequencing platforms	Illumina®, MGISEQ™ and Ion Torrent™ platforms

See the data

Pillar technology

Pillar provides a seamlessly integrated NGS workflow, from panel design to data analysis.

ampPD™

Primer design platform for reliable amplicon specificity

SLIMamp®

Enrichment chemistry that enables single-tube amplification

PiVAT®

Bioinformatic tools that deliver sensitive, robust variant calls

Ordering information

Panel	Part Number
ONCO/Reveal BRCA1 & BRCA2 plus CNV Panel	HDA-BR-1003-24

Plus

Pillar Index Kit Options	Part Number
Pillar Kit A, indices PI501-8, PI701-4 (32 combinations)	IDX-PI-1001-96
Pillar Kit B, indices PI501-8, PI705-8 (32 combinations)	IDX-PI-1002-96
Pillar Kit C, indices PI501-8, PI709-12 (32 combinations)	IDX-PI-1003-96
Pillar Kit D, indices PI501-8, PI701-12 (96 combinations)	IDX-PI-1004-192
Pillar Kit E, indices PI501-8, PI701-12 (96 combinations)	IDX-PI-1004-384

or

Ion Torrent Index Kit Options	Part Number
Pillar Biosciences IonXpress Indexing Kit A (24 combinations) includes ION Common Index	IDX-TI-1001-96

or

MGI Index Kit Options	Part Number
Pillar Biosciences MGI Indexing Kit A (24 combinations) includes BGI Common Index	IDX-BI-1001-96