Pillar® ONCO/Reveal™ BRCA1 & BRCA2 plus CNV Panel
The ONCO/Reveal BRCA1 & BRCA2 plus CNV Panel is designed for the detection of exon-level copy number variation (CNV) across the entire coding region of the BRCA1 and BRCA2 genes (as well as 10bp flanking introns). The assay is designed with shorter amplicons to allow for detection of mutations from FFPE samples, while also being compatible with intact genomic DNA from blood samples.
Panel highlights
Full BRCA1 and BRCA2 CDS plus flanking sequences
Detects exon level CNVs, SNVs, and indels
Detection down to 1% VAF even with low sample input/quality
Robust to FFPE degradation
For research use only. Not for use in diagnostic procedures.
Panel specifications
Enrichment chemistry | Multiplex PCR using tiled amplicons |
Number of pools | 1 pool |
Number of genes/amplicons | 2/283 |
Number of targets | Full coding gene sequence (CDS) with flanking intronic regions (10bp) |
Variant types | Exon level CNV, SNV, small and medium indels |
Average amplicon size | 155 bp |
Recommended DNA input range | 20 ng to 80 ng |
Sample types | DNA from tissue or blood; FFPE |
Mapping rate | 99.0% ± 0.3% |
% on-target aligned reads | 98.6% ± 0.2% |
Coverage uniformity (% targets with >0.2X mean coverage) | 98.0% ± 0.1% |
Total assay time (from DNA to sequencer) | <8 hours |
Sequencing platforms | Illumina®, MGISEQ™ and Ion Torrent™ platforms |
See the data

Pillar technology
Pillar provides a seamlessly integrated NGS workflow, from panel design to data analysis.
Ordering information
Panel | Part Number |
---|---|
ONCO/Reveal BRCA1 & BRCA2 plus CNV Panel | HDA-BR-1003-24 |
Plus
Pillar Index Kit Options | Part Number |
---|---|
Pillar Kit A, indices PI501-8, PI701-4 (32 combinations) | IDX-PI-1001-96 |
Pillar Kit B, indices PI501-8, PI705-8 (32 combinations) | IDX-PI-1002-96 |
Pillar Kit C, indices PI501-8, PI709-12 (32 combinations) | IDX-PI-1003-96 |
Pillar Kit D, indices PI501-8, PI701-12 (96 combinations) | IDX-PI-1004-192 |
Pillar Kit E, indices PI501-8, PI701-12 (96 combinations) | IDX-PI-1004-384 |
or
Ion Torrent Index Kit Options | Part Number |
---|---|
Pillar Biosciences IonXpress Indexing Kit A (24 combinations) includes ION Common Index | IDX-TI-1001-96 |
or
MGI Index Kit Options | Part Number |
---|---|
Pillar Biosciences MGI Indexing Kit A (24 combinations) includes BGI Common Index | IDX-BI-1001-96 |
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