Pillar® ONCO/Reveal™ BRCA1 & BRCA2 plus CNV Panel

The ONCO/Reveal BRCA1 & BRCA2 plus CNV Panel is designed for the detection of exon-level copy number variation (CNV) across the entire coding region of the BRCA1 and BRCA2 genes (as well as 10bp flanking introns). The assay is designed with shorter amplicons to allow for detection of mutations from FFPE samples, while also being compatible with intact genomic DNA from blood samples.

Panel highlights

Full BRCA1 and BRCA2 CDS plus flanking sequences

Detects exon level CNVs, SNVs, and indels

Detection down to 1% VAF even with low sample input/quality

Robust to FFPE degradation

For research use only. Not for use in diagnostic procedures.

Panel specifications

Enrichment chemistry Multiplex PCR using tiled amplicons
Number of pools 1 pool
Number of genes/amplicons 2/283
Number of targets Full coding gene sequence (CDS) with flanking intronic regions (10bp)
Variant types Exon level CNV, SNV, small and medium indels ​
Average amplicon size​ 155 bp
Recommended DNA input range 20 ng to 80 ng
Sample types DNA from tissue or blood; FFPE
Mapping rate 99.0% ± 0.3%
% on-target aligned reads 98.6% ± 0.2%
Coverage uniformity ​ (% targets with >0.2X mean coverage) 98.0% ± 0.1%
Total assay time (from DNA to sequencer) <8 hours
Sequencing platforms​ Illumina®, MGISEQ™ and Ion Torrent™ platforms

See the data

Copy number across all amplicons covering the entire BRCA2 coding sequence figure

Pillar technology

Pillar provides a seamlessly integrated NGS workflow, from panel design to data analysis.

ampPD™

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Primer design platform for reliable amplicon specificity

SLIMamp®

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Enrichment chemistry that enables single-tube amplification

PiVAT®

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Bioinformatic tools that deliver sensitive, robust variant calls

Ordering information

Panel Part Number
ONCO/Reveal BRCA1 & BRCA2 plus CNV Panel HDA-BR-1003-24​

Plus

Pillar Index Kit Options Part Number
Pillar Kit A, indices PI501-8, PI701-4 (32 combinations)​​ IDX-PI-1001-96​​
Pillar Kit B, indices PI501-8, PI705-8 (32 combinations)​ IDX-PI-1002-96​​
Pillar Kit C, indices PI501-8, PI709-12 (32 combinations)​ IDX-PI-1003-96​
Pillar Kit D, indices PI501-8, PI701-12 (96 combinations)​ ​​IDX-PI-1004-192​
Pillar Kit E, indices PI501-8, PI701-12 (96 combinations)​ IDX-PI-1004-384​

or

Ion Torrent Index Kit Options​​ Part Number
Pillar Biosciences IonXpress Indexing Kit A (24 combinations) includes ION Common Index​ IDX-TI-1001-96​

or

MGI Index Kit Options​​ Part Number
Pillar Biosciences MGI Indexing Kit A (24 combinations) includes BGI Common Index​ IDX-BI-1001-96​

Contact an expert

Contact us for technical information or to get a quote

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