PCR multiplexing starts with well-designed primers

ampPD™ automates primer design for overlapping multiplex PCR workflows

The Pillar® ampPD primer design pipeline proactively masks all regions where primers cannot bind, automatically identifies potential primer-dimers, and suggests the optimal location for primer placement for each and every amplicon. 

With primers designed in ampPD, you can selectively amplify targets from minimal input in a single tube. ampPD is the starting point for all of our off-the-shelf panels and enables custom design for genomic targets that fit your panel criteria.

 

Rapid

  • Perform tunable masking for multi-copy regions, repeats and variants.
  • Delineate regions of interest (ROI) and collapse proximal ROIs.
graphic showing masking of unprimable regions

Flexible

  • Exhaustively calculate all primer tiling solutions for each ROI.
  • Select primers that are optimized for any sample including FFPE.
Amplicons around the region of interest

Robust

  • Calculate potential amplifiable dimer pairs via cluster-based computing.
  • Compute single pool with the lowest dimer potential.
Network graphic

Primer-primer interaction map 

ampPD was built to go hand-in-hand with Pillar’s SLIMamp® chemistry and PiVAT® bioinformatics platform–so the entire NGS workflow is seamlessly integrated, even for custom genomic targets.

ampPD™

icon of ampPD primer as located on DNA

Primer design platform for reliable amplicon specificity

SLIMamp®

test tube icon

Enrichment chemistry that enables single-tube amplification

PiVAT®

PiVat icon

Bioinformatic tools that deliver sensitive, robust variant calls

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