PCR multiplexing starts with well-designed primers
ampPD™ automates primer design for overlapping multiplex PCR workflows
The Pillar® ampPD primer design pipeline proactively masks all regions where primers cannot bind, automatically identifies potential primer-dimers, and suggests the optimal location for primer placement for each and every amplicon.
With primers designed in ampPD, you can selectively amplify targets from minimal input in a single tube. ampPD is the starting point for all of our off-the-shelf panels and enables custom design for genomic targets that fit your panel criteria.
Rapid
- Perform tunable masking for multi-copy regions, repeats and variants.
- Delineate regions of interest (ROI) and collapse proximal ROIs.
Flexible
- Exhaustively calculate all primer tiling solutions for each ROI.
- Select primers that are optimized for any sample including FFPE.
Robust
- Calculate potential amplifiable dimer pairs via cluster-based computing.
- Compute single pool with the lowest dimer potential.
Primer-primer interaction map
ampPD was built to go hand-in-hand with Pillar’s SLIMamp® chemistry and PiVAT® bioinformatics platform–so the entire NGS workflow is seamlessly integrated, even for custom genomic targets.
ampPD™
Primer design platform for reliable amplicon specificity
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